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spy555 actin probe  (Cytoskeleton Inc)


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    Structured Review

    Cytoskeleton Inc spy555 actin probe
    Spy555 Actin Probe, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spy555+actin/SPY555-Actin/pm42703967-96-5-7
    Average 95 stars, based on 34 article reviews
    spy555 actin probe - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    other:

    Article Title: Directional guidance to orient Schwann cell alignment in nerve regeneration requires Plexin-B1
    Article Snippet: Nerve sections or whole-mount preparations were incubated overnight at 37°C and then imaged using a Zeiss Axioscope (sections) or Nikon (whole mounts) microscope.

    Control:

    Article Title: Mammalian TMC1 or 2 are necessary for scramblase activity in auditory hair cells
    Article Snippet: .. For each mouse, one cochlea was treated as a control, and incubated in HBSS containing 1:25 annexin V–Alexa Fluor 647 (Thermo Fisher Scientific), and spy555-actin (Cytoskeleton, Inc.) for actin filaments visualization. ..

    Incubation:

    Article Title: Mammalian TMC1 or 2 are necessary for scramblase activity in auditory hair cells
    Article Snippet: .. For each mouse, one cochlea was treated as a control, and incubated in HBSS containing 1:25 annexin V–Alexa Fluor 647 (Thermo Fisher Scientific), and spy555-actin (Cytoskeleton, Inc.) for actin filaments visualization. ..

    Article Title: The Extracellular Matrix Regulates Invasion in Fusion-Negative Rhabdomyosarcoma via YAP-PIEZO1 Signaling Axis.
    Article Snippet: .. For each chamber, 500 μL culture media containing 2 μL Fluo-4 AM (abcam, Waltham, MA, USA, cat. Ab241082) and 1 μL SPY555-Actin (Cytoskeleton, Denver, CO, USA, cat. CY-SC202) was added to cultured cells and incubated for 1 h. If drug treatment was performed at this step, drugs were added and diluted to working concentration in the media prior to the addition of dyes. .. After incubation, the staining solution was discarded, cells were washed twice with PBS, and 500 uL of culture media without pheno-red (DMEM containing 10% FBS and 1% penicillin/streptomycin) was added to each well and imaged on a Nikon inverted fluorescent microscope.

    Article Title: The Extracellular Matrix Regulates Invasion in Fusion-Negative Rhabdomyosarcoma via YAP–PIEZO1 Signaling Axis
    Article Snippet: .. For each chamber, 500 μL culture media containing 2 μL Fluo-4 AM (abcam, Waltham, MA, USA, cat. Ab241082) and 1 μL SPY555-Actin (Cytoskeleton, Denver, CO, USA, cat. CY-SC202) was added to cultured cells and incubated for 1 h. If drug treatment was performed at this step, drugs were added and diluted to working concentration in the media prior to the addition of dyes. .. After incubation, the staining solution was discarded, cells were washed twice with PBS, and 500 uL of culture media without pheno-red (DMEM containing 10% FBS and 1% penicillin/streptomycin) was added to each well and imaged on a Nikon inverted fluorescent microscope.

    Staining:

    Article Title: Polarization increases nuclear stiffness in macrophages despite reduction in lamin A/C levels
    Article Snippet: Secondary antibodies used were Alexa Fluor 488, 568, or 647-conjugated donkey anti mouse or rabbit-IgG antibodies (Invitrogen). .. Cells were stained with Spy555- Actin (Cytoskeleton, CY-SC202) at 1:1000 dilution in PBS and DAPI (Thermo Fisher Scientific, 62247) at 1:500 dilution in PBS at room temperature for 1 h. Samples were washed 3 times for 5 min each in PBS and left in PBS + 0.02% sodium azide in the dark until imaging. ..

    Imaging:

    Article Title: Polarization increases nuclear stiffness in macrophages despite reduction in lamin A/C levels
    Article Snippet: Secondary antibodies used were Alexa Fluor 488, 568, or 647-conjugated donkey anti mouse or rabbit-IgG antibodies (Invitrogen). .. Cells were stained with Spy555- Actin (Cytoskeleton, CY-SC202) at 1:1000 dilution in PBS and DAPI (Thermo Fisher Scientific, 62247) at 1:500 dilution in PBS at room temperature for 1 h. Samples were washed 3 times for 5 min each in PBS and left in PBS + 0.02% sodium azide in the dark until imaging. ..

    Cell Culture:

    Article Title: The Extracellular Matrix Regulates Invasion in Fusion-Negative Rhabdomyosarcoma via YAP-PIEZO1 Signaling Axis.
    Article Snippet: .. For each chamber, 500 μL culture media containing 2 μL Fluo-4 AM (abcam, Waltham, MA, USA, cat. Ab241082) and 1 μL SPY555-Actin (Cytoskeleton, Denver, CO, USA, cat. CY-SC202) was added to cultured cells and incubated for 1 h. If drug treatment was performed at this step, drugs were added and diluted to working concentration in the media prior to the addition of dyes. .. After incubation, the staining solution was discarded, cells were washed twice with PBS, and 500 uL of culture media without pheno-red (DMEM containing 10% FBS and 1% penicillin/streptomycin) was added to each well and imaged on a Nikon inverted fluorescent microscope.

    Article Title: The Extracellular Matrix Regulates Invasion in Fusion-Negative Rhabdomyosarcoma via YAP–PIEZO1 Signaling Axis
    Article Snippet: .. For each chamber, 500 μL culture media containing 2 μL Fluo-4 AM (abcam, Waltham, MA, USA, cat. Ab241082) and 1 μL SPY555-Actin (Cytoskeleton, Denver, CO, USA, cat. CY-SC202) was added to cultured cells and incubated for 1 h. If drug treatment was performed at this step, drugs were added and diluted to working concentration in the media prior to the addition of dyes. .. After incubation, the staining solution was discarded, cells were washed twice with PBS, and 500 uL of culture media without pheno-red (DMEM containing 10% FBS and 1% penicillin/streptomycin) was added to each well and imaged on a Nikon inverted fluorescent microscope.

    Concentration Assay:

    Article Title: The Extracellular Matrix Regulates Invasion in Fusion-Negative Rhabdomyosarcoma via YAP-PIEZO1 Signaling Axis.
    Article Snippet: .. For each chamber, 500 μL culture media containing 2 μL Fluo-4 AM (abcam, Waltham, MA, USA, cat. Ab241082) and 1 μL SPY555-Actin (Cytoskeleton, Denver, CO, USA, cat. CY-SC202) was added to cultured cells and incubated for 1 h. If drug treatment was performed at this step, drugs were added and diluted to working concentration in the media prior to the addition of dyes. .. After incubation, the staining solution was discarded, cells were washed twice with PBS, and 500 uL of culture media without pheno-red (DMEM containing 10% FBS and 1% penicillin/streptomycin) was added to each well and imaged on a Nikon inverted fluorescent microscope.

    Article Title: The Extracellular Matrix Regulates Invasion in Fusion-Negative Rhabdomyosarcoma via YAP–PIEZO1 Signaling Axis
    Article Snippet: .. For each chamber, 500 μL culture media containing 2 μL Fluo-4 AM (abcam, Waltham, MA, USA, cat. Ab241082) and 1 μL SPY555-Actin (Cytoskeleton, Denver, CO, USA, cat. CY-SC202) was added to cultured cells and incubated for 1 h. If drug treatment was performed at this step, drugs were added and diluted to working concentration in the media prior to the addition of dyes. .. After incubation, the staining solution was discarded, cells were washed twice with PBS, and 500 uL of culture media without pheno-red (DMEM containing 10% FBS and 1% penicillin/streptomycin) was added to each well and imaged on a Nikon inverted fluorescent microscope.



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    Effects of LPS-CM on SCAP apoptosis and senescence. A . Apoptosis rate detected by flow cytometry. Q2-1: dead cells, Q2-2: late apoptotic cells. Q2-3: live cells; Q2-4: early apoptotic cells; B . Statistical summary of apoptosis rate of SCAP, n =4; C , D . Western blot analysis of senescence-related proteins P21 and P53, n =3; E, F. Representative images and quantitative analysis of SA β-gal staining, scale bar 200 μm, n =5, ** P < 0.01, ns no statistical significance; G, H . Immunofluorescence staining and quantitative analysis of P53 under <t>LPS-CM.</t> <t>Cytoskeleton-Actin</t> (red) and DAPI (blue) were chosen to co-marker the morphology and location, P53 (Green) was expressed in the nucleus, and its fluorescence intensity was significantly increased in the LPS-10 CM group, scale bar 5 μm, n =5
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    Image Search Results


    Effects of LPS-CM on SCAP apoptosis and senescence. A . Apoptosis rate detected by flow cytometry. Q2-1: dead cells, Q2-2: late apoptotic cells. Q2-3: live cells; Q2-4: early apoptotic cells; B . Statistical summary of apoptosis rate of SCAP, n =4; C , D . Western blot analysis of senescence-related proteins P21 and P53, n =3; E, F. Representative images and quantitative analysis of SA β-gal staining, scale bar 200 μm, n =5, ** P < 0.01, ns no statistical significance; G, H . Immunofluorescence staining and quantitative analysis of P53 under LPS-CM. Cytoskeleton-Actin (red) and DAPI (blue) were chosen to co-marker the morphology and location, P53 (Green) was expressed in the nucleus, and its fluorescence intensity was significantly increased in the LPS-10 CM group, scale bar 5 μm, n =5

    Journal: BMC Oral Health

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    Figure Lengend Snippet: Effects of LPS-CM on SCAP apoptosis and senescence. A . Apoptosis rate detected by flow cytometry. Q2-1: dead cells, Q2-2: late apoptotic cells. Q2-3: live cells; Q2-4: early apoptotic cells; B . Statistical summary of apoptosis rate of SCAP, n =4; C , D . Western blot analysis of senescence-related proteins P21 and P53, n =3; E, F. Representative images and quantitative analysis of SA β-gal staining, scale bar 200 μm, n =5, ** P < 0.01, ns no statistical significance; G, H . Immunofluorescence staining and quantitative analysis of P53 under LPS-CM. Cytoskeleton-Actin (red) and DAPI (blue) were chosen to co-marker the morphology and location, P53 (Green) was expressed in the nucleus, and its fluorescence intensity was significantly increased in the LPS-10 CM group, scale bar 5 μm, n =5

    Article Snippet: Cytoskeleton-Actin (red) and DAPI (blue) were chosen to co-marker the morphology and location, P53 (Green) was expressed in the nucleus, and its fluorescence intensity was significantly increased in the LPS-10 CM group, scale bar 5 μm, n =5

    Techniques: Flow Cytometry, Western Blot, Staining, Immunofluorescence, Marker, Fluorescence